Results of Human Genome Project have brought researchers closer to understand the relationship between individual genetic constitution and drug response differences among populations, as well as diseases susceptibility.
The ability to detect highly predictive genotype-phenotype associations may be useful for several applications, from physicians (to identify the best therapy), epidemiologists (for association studies) till researchers (involved in new drug development).

Only in the year 2010, pharmacogenetics and SNPs topics have been cited respectively in over than 5000 and 700 publications in the NCBI database. Pharmacogenetics is evolved from a niche discipline to a major driving force in clinical pharmacology and currently is one of the most actively pursued disciplines in applied biomedical research [Jürgen Brockmöller & Mladen V. Tzvetkov, 2008].
Pharmacogenetics has changed the preclinical and clinical practices in drug research and, today, large clinical trials without a pharmacogenetic add-on appear to have become the minority [Jürgen Brockmöller & Mladen V. Tzvetkov, 2008].
Moreover, the advances in high-throughput genotyping technologies are poised to provide a flood of information that will affect both pharmacogenetic discovery and pharmacogenetic application in clinical practice.

Although direct DNA sequencing is considered as a "gold standard" for genotyping of SNPs and mutations, it still remains relatively expensive, laborious and time consuming [Patricia A. et al 2009]. Different methods have been developed to simplify the detection of novel mutations, from RFLP to SSCP (single strand conformation polymorphism) and DGGE (denaturing gradient gel electrophoresis), DHPLC (denaturing high performance liquid chromatography), DNA microarray, and finally several PCR based methods (allele/specific amplification, single-base extension etc).
High Resolution Melting (HRM) is a simple, rapid and low cost mutation scanning method. Its advantage is that PCR amplification and melting curve analysis perform within the same tube or plate, without any post-PCR processing. This method (feature) is particularly important for a routine diagnostic setting [Patricia A. et al 2009].

In order to simplify and speed up researchers and technicians work, the HRM kits from DIATHEVA are made to provide a flexible platform that allows simultaneous detection of several SNPs in order to economize as much as possible both time and manual labour and to minimize the needs of results interpretation. All HRM Kits from DIATHEVA work at the same operating conditions, enabling clustering of several SNPs and their simultaneous detection.

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